Nikoline Nielsen (born October 3, 1987) is a Danish left back handballer, playing for Slagelse FH.
She became a league player, when she changed from the Danish 1st division club Lyngby HK in 2007 to GOG. When they merged with Odense hf to Odense GOG (becoming HC Odense) she continued there. Before Lyngby HK she played for Ålhom IF and Hillerød.
She began on the Danish national team October 16, 2007, and as of March 12, 2011, she has played nine games and scored 11 goals. After being out in two years because of an injury, she was selected for the national team again in August 2010.