Sir John Bourne (by 1518-1575), of Worcestershire, was an English politician.
Bourne's family were minor gentry from Worcestershire. He married Dorothy Lygon and they had two sons and three daughters.
He was educated at Lincoln's Inn and was knighted on 2 October 1553. He was a Member (MP) of the Parliament of England for Guildford in 1539, Midhurst in 1542, Preston in 1545, Worcester in October 1553, and Worcestershire in April 1554, November 1554, 1555 and 1558.