Eva Puskarčíková (Czech pronunciation: [ˈɛva ˈpuskartʃiːkovaː], born 3 January 1991) is a Czech biathlete. Her best result in the Biathlon World Cup is 3rd place in the pursuit in the 2016–17 season. She competed at the 2014 Winter Olympics in Sochi. Her best result here was 4th place in relay, shared with her Czech teammates Gabriela Soukalová, Jitka Landová and Veronika Vítková. Apart from that, she also competed in sprint, pursuit and individual.