Basilius Besler (1561–1629) was a respected Nuremberg apothecary and botanist, best known for his monumental Hortus Eystettensis. He was curator of the garden of Johann Konrad von Gemmingen, prince bishop of Eichstätt in Bavaria. The bishop was an enthusiastic botanist who derived great pleasure from his garden, which rivaled Hortus Botanicus Leiden among early European botanical gardens outside Italy.
The gardens surrounded the bishop's palace, Willibaldsburg, which was built on a hill overlooking the town. These gardens had been started in 1596 and designed by Besler's colleague, Joachim Camerarius the Younger (1534–1598), a physician and botanist. Upon Camerarius' death in 1598, Besler had the remainder of Camerarius' plants moved to Eichstätt and carried on the work of planting and supervision. The bishop commissioned Besler to compile a codex of the plants growing in his garden, a task which Besler took sixteen years to complete, the bishop dying shortly before the work was published.
The gardens were sacked by invading Swedish troops under Herzog Bernhard von Weimar in 1633-4, but were reconstructed and opened to the public of Eichstätt in 1998.