Maria Machteld van Sypesteyn (1724 – 1774) was a painter from the Netherlands.
Sypesteyn was born in Haarlem as the daughter of the mayor Cornelis Ascanius van Sypesteyn (1694-1744), baljuw van Brederode, and Maria de Lange (1696-1744). She became a pupil of the Haarlem miniature painter Henriëtte Wolters-van Pee, who she probably met through her father, who was a regent of the Proveniershuis where Henrëtte lived. She is known for miniatures on ivory in the manner of her teacher. She married the wealthy mr. Pieter van Schuylenburch, heer van Moermont en Renesse (1714-1764) on 9 July 1743 in The Hague and the couple lived in Haarlem, where her husband became mayor in the 1760s. He died relatively young in 1764 and was buried in the family grave in the St. Bavochurch. In 1767 she remarried another member of the Haarlem regency, mr. David van Lennep, who contracted Jurriaan Andriessen to paint new wall decorations in his Huis te Manpad for her.
Sypesteyn died in Heemstede and was buried in the St. Bavochurch. Most of her works are still in the family collection.